CytExpert Software for the CytoFLEX Platform

CytExpert is a highly capable software program that controls instrument operation, and data collection and analysis. Novice to experienced flow cytometrists can learn to operate the system quickly, confidently set up experiment based protocols and export publication quality data.

  • Default installation option requires no user login
  • User Management installation option contains features for user and role management
  • Electronic Records Management installation option provides tools for compliance to 21 CFR Part 11

View release note for the latest version


Acquisition Functions

CytExpert Acquisition Functions 
  1. Acquisition Setting: Select the desired acquisition settings under the Acq. Setting tab. Settings may be imported from an FCS file or from the Settings Catalog. 
  2. Channel: Select the channels and create label names under the Channel tab.
  3. Compensation Matrix: Set compensation under the Compensation Matrix tab.
  4. Stopping Rules: Select Events to record, time to record, or volume to record under the Stopping Rules tab.
  5. Custom Metadata: enter up to ten sample identifiers manually. Alternatively, import into a Plate Layout from a .csv file.


Analysis Features

Plot Types Gates Statistics Compensation
  • Histograms
  • Dot Plots
  • Density Plots
  • Contour Plots
  • Overlay Plots
  • Pseudo Color Plots
  • Heat Map
  • Lasso
  • Polygon
  • Rectangle
  • Four-quadrant
  • Hinged
  • Auto polygon 
  • Number
  • % Total
  • % Gated
  • % Grandparent
  • Median
  • Arithmetic Mean
  • Mode
  • Standard Deviation (SD)
  • Coefficient of Variation (CV)
  • Absolute Count
  • Adjusting Compensation
  • Saving a Compensation File
  • Saving a Compensation Library
  • Importing a Compensation File
  • Accounting for Autofluorescence 


Minimum Computer Requirements

Required processor 4th Gen Intel® Core™ i3 (3 MB Cache, 2.90 GHz) equivalent or above
Required memory 4 GB RAM or above
Required hard disk space At least 1 G free space for the disk of the experiment for analysis. Recommend 10 G for data acquisition.
Required display 1920 x 1080 resolution for optimal display
USB Port USB 2.0 or above for data acquisition


CytExpert software request