Exquisite Sensitivity

Harness the Power of Advanced Sensitivity

A unique assembly of technologies contributes to the exquisite sensitivity of the platform. Borrowing technology from the telecommunications industry, the Wavelength Division Multiplexer (WDM) deconstructs and measures multiple wavelengths of light. The WDM relies on fiber optics and band pass filters to separate the light wavelengths. Unlike more traditional instruments, multiple dichroic filters to direct the light path are not required. This makes it much easier to configure the fluorescence channels, but also increases light efficiency as light loss due to refraction is minimized.

 

The WDM utilizes Avalanche Photodiode detectors (APD), versus Photomultiplier tubes (PMT). One hallmark of the photodiode is the high quantum efficiency in excess of 80%, especially for wavelengths greater than 800 nm.

With conventional analyzers, laser excitation sources are optimized by shaping and focusing light through a series of lenses and filters onto the flow cell. Each of these light interactions is an opportunity for light loss. Another component of the system which increases efficiency is the use of integrated optics to focus light onto the flow cell. All of these technologies work together in the CytoFLEX to ensure efficient light management for optimal excitation and emission of fluorochrome-tagged cells, which is critical to its performance.

The graphics below demonstrate excellent resolution of all peaks using SPHERO™ Rainbow 8-peak beads or SPHERO™ Fluorescent IR Flow Cytometer Particles.

 

355 nm UV Laser Channels
Spherotech 8-peak bead data using CytoFLEX 355 nm laser excitation and 405/30 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 355 nm laser excitation and 525/40 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 355 nm laser excitation and 675/30 nm bandpass filter
375 nm Near UV Laser Channels
Spherotech 8-peak bead data using CytoFLEX 375 nm laser excitation and 450/45 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 375 nm laser excitation and 525/40 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 375 nm laser excitation and 675/30 nm bandpass filter
405 nm Violet Laser Channels
Spherotech 8-peak bead data using CytoFLEX 405 nm laser excitation and 450/45 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 405 nm laser excitation and 525/40 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 405 nm laser excitation and 610/20 nm bandpass filter
Spherotech 8-peak bead data using CytoFLEX 405 nm laser excitation and 660/10 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 405 nm laser excitation and 763/43 nm bandpass filter  
488 nm Blue Laser Channels
Spherotech 8-peak bead data using CytoFLEX 488 nm laser excitation and 525/40 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 488 nm laser excitation and 585/42 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 488 nm laser excitation and 610/20 nm bandpass filter
Spherotech 8-peak bead data using CytoFLEX 488 nm laser excitation and 690/50 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 488 nm laser excitation and 780/60 nm bandpass filter  
561 nm Yellow Green Laser Channels
Spherotech 8-peak bead data using CytoFLEX 561 nm laser excitation and 585/42 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 561 nm laser excitation and 610/20 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 561 nm laser excitation and 675/30 nm bandpass filter
Spherotech 8-peak bead data using CytoFLEX 561 nm laser excitation and 710/50 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 561 nm laser excitation and 763/43 nm bandpass filter
638 nm Red Laser Channels
Spherotech 8-peak bead data using CytoFLEX 638 nm laser excitation and 660/10 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 638 nm laser excitation and 712/25 nm bandpass filter Spherotech 8-peak bead data using CytoFLEX 638 nm laser excitation and 763/43 nm bandpass filter
808 nm Infrared Laser Channels
SPHERO Fluoroescent IR Flow Cytometry particle data using CytoFLEX 808 nm laser excitation and 840/20 nm bandpass filter SPHERO Fluoroescent IR Flow Cytometry particle data using CytoFLEX 808 nm laser excitation and 885/40 nm bandpass filter  

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